Analysis of Compound Content of Ethanol Extract of Butterfly Pea Flower ( Clitoria ternatea L.) Using Thin Layer Chromatography Method: A Systematic Literature Review

The butterfly flower ( Clitoria ternatea L.) is an herbal plant that is widely used in traditional medicine in Indonesia. The chemical compounds in butterfly pea flowers are thought to have various biological activities, such as antioxidant, anti-inflammatory, antibacterial and antidiabetic. Analysis of the compound content in butterfly pea flower extract can be done using various methods, one of which is thin layer chromatography (TLC). The literature search process was carried out on various databases (PubMed, Web of Sciences, EMBASE, Cochrane Libraries, and Google Scholar) analysis of the compound content of ethanol extract of butterfly pea flowers using the thin layer chromatography method. This study follows the preferred reporting items for systematic reviews and meta-analysis (PRISMA) recommendations. The ethanol extract of butterfly pea flowers contains various groups of secondary metabolite compounds, such as flavonoids, saponins, terpenoids and tannins. These compounds are thought to have various biological activities, such as antioxidant, anti-inflammatory, antibacterial and antidiabetic.


Introduction
The butterfly pea flower is an herbal plant that originated in South America but has now spread to various parts of the world, including Indonesia.This plant has the scientific name Clitoria ternatea L. and belongs to the Fabaceae family.Butterfly pea flowers have various benefits.As a food plant, butterfly pea flowers can be processed into various kinds of food and drinks, such as tea, syrup, and ice cream.As a natural dye, butterfly pea flowers can be used to color food, drinks, and fabrics.As a traditional medicine, butterfly pea flowers have various benefits, such as Increasing body endurance, reducing inflammation, helping lower blood sugar levels, helping overcome insomnia, and helping to overcome stress.The chemical compounds in butterfly pea flowers are thought to play a role in various biological activities.
These compounds include flavonoids, such as quercetin and kaempferol, which have antioxidant, anti-inflammatory, and antibacterial properties.
Saponins, such as triterpenoid saponins and steroid saponins, have anti-inflammatory, antibacterial, and antifungal activities.
[3] Thin layer chromatography (TLC) is a method of separating compounds based on polarity differences.
This method is carried out by dripping the sample solution onto a TLC plate that has been coated with a stationary phase.The stationary phase most often used in TLC is silica gel.Then, the TLC plate is inserted into a vessel containing the mobile phase.The mobile phase used must have a different polarity from the stationary phase.The mobile phase most often used in TLC is a mixture of organic solvents.
Compounds in the sample solution will move up the TLC plate at different speeds, depending on their polarity.More polar compounds will move slower, while less polar compounds will move faster.After the TLC plate is dried, the separated compounds can be observed using ultraviolet (UV) light or a dye.TLC is a simple, easy, and relatively cheap method.This method can be used to separate various types of compounds, including compounds contained in butterfly pea flower extract. 4-6

Methods
The

Conclusion
The ethanol extract of butterfly pea flowers contains various groups of secondary metabolite compounds, such as flavonoids, saponins, terpenoids, and tannins.These compounds are thought to have various biological activities, such as antioxidant, antiinflammatory, antibacterial, and antidiabetic.